Brian Casey bogeyed two of his last three holes and failed to get through the First Stage of the European Tour Qualifying School at Stoke by Nayland.
The 26-year-old from Headfort finished three strokes outside the top 20 and ties in a share of 24th on level par after a closing 71.
Old Conna's Neil O'Briain birdied two his last three but also shot 71 to finish a shot further back on one-over while Peter Williamson shot 76 to share 57th on eight-over.
Exactly 20 players finished on three-under-par or better with American Steven Burak topping the leaderboard by five strokes on 14-under-par from Swede Gabriel Axell.
There were no Irish in action at the other Section B First Stage qualifier at Golfclub Schloss Ebreichsdorf in Austria where 22 players qualified on four-under or better headed by Argentina's Tommy Cocha, who won by five on 15-under.
The third round of four First Stage (Section C) qualifiers take place at Golf d’Hardelot in France and Ribagolfe in Portugal from Tuesday.